Research Associate I / II - Research Associate - Cell therapy / Marson Lab

Company: Gladstone Institutes
Location: San Francisco
Posted on: October 18, 2024

Job Description:

Research Associate I / II - Research Associate - Cell therapy / Marson Lab
About the role:
The Gladstone-UCSF Institute of Genomic Immunology is recruiting a Research Associate (RA) to help perform studies related to genetic engineering and functional assessment of T cell therapies, primarily CAR-T cells. Our institute has pioneered the application of novel genetic engineering technologies such as CRISPR to primary human immune cells. These new technical abilities have furthered our research into the genetic control of T cell biology as well as opened the door to novel cancer immunotherapies based on engineered human immune cells. This individual would be part of a preclinical research team under the direction of Justin Eyquem (UCSF) streamlining the generation, benchmarking, and functional assessment of genetically engineered human T cell therapies for cancer and inflammatory disease.
What you will do:

• 
  
• The RA will utilize various protocols to generate the molecular components, such as DNA constructs, gRNA library, lentiviral vectors and Adeno-Associated Vectors necessary for specific cellular engineering experiments.
  
• Isolation and culture of primary human T cells, as well as functional analysis of engineered cells via flow cytometry, cellular assays, Sanger sequencing, next-generation DNA sequencing, Single-Cell RNA sequencing.
  
• Design and completion of cellular editing experiments, as well as in vitro analysis of novel cellular cancer immunotherapies and coordination with the in vivo team for mouse experiments employing the T cell products under development.
  
  What you will need:
  
  • 
    
  • BS or BA or MS 2+ years related experience in cellular Biology, immunology, or related field; or an equivalent combination of education and experience.
    
  • New graduates with a commitment to learning are welcome to apply.
    
  • Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to DNA and RNA isolation, PCR, gel electrophoresis, DNA assembly, bacterial transformation, mammalian cell culture, etc.).
    
  • Experience with T cell engineering using viral and non-viral gene editing technologies, including retroviral vectors, AAV, RNA/RNP electroporation and analysis via multicolor flow cytometry.
    
  • Experience with in vitro characterization of engineered T cells to evaluate cytotoxicity, proliferation, cytokine production and phenotype.
    
  • Experience with animal studies, including basic animal care, tumor monitor and assessment is a plus.
    
  • Experience with library design, screening and NGS sequencing is a plus.
    
  • Excellent attention to detail, organizational and interpersonal communication skills (verbal and written).
    
  • Willingness and ability to learn new methods and proactive at troubleshooting and optimization.
    
  • Ability to work independently and as a member of a research team.
    
  • Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines.
    
  • Ability to comply with policies and procedures.
    
  • Some evening and weekend hours are required.
    
    What Gladstone will offer you:
    
    • 
      
    • People -work with talented, committed, and supportive teammates within an organization that values each member of its community.
      
    • A meaningful place to grow and learn -whether it is your professional skills or scientific knowledge, we have the resources and environment to advance either.
      
    • Healthy work/life balance -you are highly engaged and productive at work because you can have time to recharge and enjoy a vibrant life outside of work.
      
    • Compensation -competitive salary. Title and salary will be commensurate with education and experience.
      
    • Excellent benefits -generous medical, dental, vision, retirement plan, paid vacation, commuter benefits, access to free shuttle transportation.
      
      If this role is not for you:
      Please stay in touch and to see a complete list of our open career opportunities, please visit:
      Hiring Range:
      
      • 
        
      • RA I $25-$29/hr.
        
      • RA II $66,560- $78,306K
        
        Gladstone Perks & Benefits:
        
        • 
          
        • People -work with talented, committed, and supportive teammates within an organization that values each member of its community.
          
        • A meaningful place to grow and learn -whether it's your professional skills or scientific knowledge, we have the resources and environment to advance either.
          
        • Healthy work/life balance -you are highly engaged and productive at work because you can have time to recharge and enjoy a vibrant life outside of work.
          
        • Compensation -competitive salary. Title and salary will be commensurate with education and experience.
          
        • Excellent benefits -generous medical, dental, vision, retirement plan, paid vacation, commuter benefits, access to free shuttle transportation.
          
          Gladstone is committed to providing equal employment opportunities to all employees and applicants for employment without regard to race, color, sex, religion, national origin, ancestry, age, marital status, medical condition, physical or mental disability, veteran status, sexual orientation, or any other non-job related characteristic. We make all employment decisions so as to further this principle of equal employment.
          About Us
          Based in San Francisco's Mission Bay neighborhood, Gladstone is an independent state-of-the-art biomedical research institution that empowers its world-class scientists to find new pathways to cures.
          #J-18808-Ljbffr

Keywords: Gladstone Institutes, Berkeley , Research Associate I / II - Research Associate - Cell therapy / Marson Lab, Healthcare , San Francisco, California